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KMID : 0370219960400040462
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Yakhak Hoeji
1996 Volume.40 No. 4 p.462 ~ p.467
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Cytotoxic Constituents from the Forsythiae Fructus against L1210 and HL60 cells
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이준성/Lee JS
민병선/배기환/Min BS/Bae KH
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Abstract
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Forsythiae Fructus was studied on cytotoxic activities for the purpose of finding out active consituents against L1210 and HL60 cells. To isolate the active ones, the methanolic extract was partitioned into water insoluble and water soluble fractions. Furthermore, the water soluble fraction was fractionated into four parts, n-hexane, benzene, ethylacetate and water fractions. Among these, the water insoluble fraction showed the most potent cytotoxic activities on L1210 and HL60 cells in vitro. The water insoluble fraction was applied to silica gel column chromatography and divided into 5 fractions(fr. 1-5). The active constituents I and II were isolated from fr.2 and 3, respectively, by repeated silica gel column chromatography and recrystallization. The constituents were identified as 3beta-acetylbetulinic acid and betulinic acid by means of physicochemical data. The ED50 values of 3beta-acetylbetulinic acid and betulinic acid were 9.10 and 16.43mcg/ml against L1210 cells and 2.72 and 2.41mcg/ml against HL60 cells, respectively.
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